Cross-phyla protein annotation by structural prediction and alignment.

ABSTRACT:

Background

Protein annotation is a major goal in molecular biology, yet experimentally determined knowledge is typically limited to a few model organisms. In non-model species, the sequence-based prediction of gene orthology can be used to infer protein identity; however, this approach loses predictive power at longer evolutionary distances. Here we propose a workflow for protein annotation using structural similarity, exploiting the fact that similar protein structures often reflect homology and are more conserved than protein sequences.

Results

We propose a workflow of openly available tools for the functional annotation of proteins via structural similarity (MorF: MorphologFinder) and use it to annotate the complete proteome of a sponge. Sponges are highly relevant for in

SUBMITTER: Ruperti F 

PROVIDER: S-EPMC10176882 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature