Protein Networks Associated with Native Metabotropic Glutamate 1 Receptors (mGlu₁) in the Mouse Cerebellum.

ABSTRACT: The metabotropic glutamate receptor 1 (mGlu₁) plays a pivotal role in synaptic transmission and neuronal plasticity. Despite the fact that several interacting proteins involved in the mGlu₁ subcellular trafficking and intracellular transduction mechanisms have been identified, the protein network associated with this receptor in specific brain areas remains largely unknown. To identify novel mGlu₁-associated protein complexes in the mouse cerebellum, we used an unbiased tissue-specific proteomic approach, namely co-immunoprecipitation followed by liquid chromatography/tandem mass spectrometry analysis. Many well-known protein complexes as well as novel interactors were identified, including G-proteins, Homer, δ2 glutamate receptor, 14-3-3 proteins, and Na/K

SUBMITTER: Mansouri M 

PROVIDER: S-EPMC10177021 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature