Modulation of Apoptosis and Autophagy by Melatonin in Juglone-Exposed Bovine Oocytes.

ABSTRACT: Melatonin, an antioxidant hormone secreted by the pineal gland, has been recognized as a regulator for numerous biological events. The deleterious effects of juglone, a polyphenolic extract of walnut trees, on embryo development has been previously reported. In the current study, we aimed to display the impact of melatonin administrated during in vitro oocyte maturation (IVM) on juglone-treated oocytes. Thus, in vitro matured oocytes were collected after 24 h post incubation with juglone in the presence or absence of melatonin. Reactive oxygen species (ROS), glutathione (GSH) content, mitochondrial distribution, and the relative abundance of mRNA transcription levels were assessed in oocytes, in addition, oocytes were in vitro fertilized to check the competency levels of oocytes to generat

SUBMITTER: El-Sheikh M 

PROVIDER: S-EPMC10177052 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature