NET Proteome in Established Type 1 Diabetes Is Enriched in Metabolic Proteins.

ABSTRACT:

Background and aims

Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by a T-cell-mediated destruction of the pancreatic insulin-producing beta cells. A growing body of evidence suggests that abnormalities in neutrophils and neutrophil extracellular trap (NET) formation (NETosis) are associated with T1D pathophysiology. However, little information is available on whether these changes are primary neutrophil defects or related to the environmental signals encountered during active disease.

Methods

In the present work, the NET proteome (NETome) of phorbol 12-myristate 13-acetate (PMA)- and ionomycin-stimulated neutrophils from people with established T1D compared to healthy controls (HC) was studied by proteomic analysis.

Results

Levels of NETosis, in

SUBMITTER: Bissenova S 

PROVIDER: S-EPMC10177393 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature