Structured Illumination Microscopy Improves Spot Detection Performance in Spatial Transcriptomics.

ABSTRACT: Spatial biology is a rapidly growing research field that focuses on the transcriptomic or proteomic profiling of single cells within tissues with preserved spatial information. Imaging-based spatial transcriptomics uses epifluorescence microscopy, which has shown remarkable results for the identification of multiple targets in situ. Nonetheless, the number of genes that can be reliably visualized is limited by the diffraction of light. Here, we investigate the effect of structured illumination (SIM), a super-resolution microscopy approach, on the performance of single-gene transcript detection in spatial transcriptomics experiments. We performed direct mRNA-targeted hybridization in situ sequencing for multiple genes in mouse coronal brain tissue sections. We evaluated spot detection perfo

SUBMITTER: Linares A 

PROVIDER: S-EPMC10177490 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature