Characterization of Duffy Binding Protein II-specific CD4⁺T cell responses in Plasmodium vivax patients.

ABSTRACT: Plasmodium vivax Duffy Binding Protein region II (PvDBPII) is a leading vaccine candidate against blood-stage vivax malaria. Anti-PvDBPII antibodies potentially block parasite invasion by inhibition of erythrocyte binding. However, knowledge of PvDBPII-specific T cell responses is limited. Here, to assess the responses of PvDBPII-specific CD4⁺T cells in natural P. vivax infection, three cross-sectional studies were conducted in recovered subjects. In silico analysis was used for potential T cell epitope prediction and selection. PBMCs from P. vivax subjects were stimulated with selected peptides and examined for cytokine production by ELISPOT or intracellular cytokine staining. Six dominant T cell epitopes were identified. Peptide-driven T cell responses showed effector memory C

SUBMITTER: Thawornpan P 

PROVIDER: S-EPMC10177721 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature