Immortalization Reversibility in the Context of Cell Therapy Biosafety.

ABSTRACT: Immortalization (genetically induced prevention of replicative senescence) is a promising approach to obtain cellular material for cell therapy or for bio-artificial organs aimed at overcoming the problem of donor material shortage. Immortalization is reversed before cells are used in vivo to allow cell differentiation into the mature phenotype and avoid tumorigenic effects of unlimited cell proliferation. However, there is no certainty that the process of de-immortalization is 100% effective and that it does not cause unwanted changes in the cell. In this review, we discuss various approaches to reversible immortalization, emphasizing their advantages and disadvantages in terms of biosafety. We describe the most promising approaches in improving the biosafety of reversibly immortalized ce

SUBMITTER: Sutyagina OI 

PROVIDER: S-EPMC10178325 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature