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Common Markers and Small Molecule Inhibitors in Golgi Studies.


ABSTRACT: In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in downstream assays to examine the consequent effect on the Golgi via microscopy and western blot after drug treatment. This chapter will be useful for researchers beginning their foray into the field of intracellular trafficking and Golgi biology.

SUBMITTER: Bui S 

PROVIDER: S-EPMC10178357 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Common Markers and Small Molecule Inhibitors in Golgi Studies.

Bui Sarah S   Stark Drew D   Li Jie J   Zhang Jianchao J   Wang Yanzhuang Y  

Methods in molecular biology (Clifton, N.J.) 20230101


In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in downstream assays to examine the consequent effect on the Golgi via microscopy and western blot after drug treatment. This chapter will be useful for researchers beginning their foray into the field of intracellular trafficking and Golgi biology. ...[more]

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