Combined Structural and Computational Study of the mRubyFT Fluorescent Timer Locked in Its Blue Form.

ABSTRACT: The mRubyFT is a monomeric genetically encoded fluorescent timer based on the mRuby2 fluorescent protein, which is characterized by the complete maturation of the blue form with the subsequent conversion to the red one. It has higher brightness in mammalian cells and higher photostability compared with other fluorescent timers. A high-resolution structure is a known characteristic of the mRubyFT with the red form chromophore, but structural details of its blue form remain obscure. In order to obtain insight into this, we obtained an S148I variant of the mRubyFT (mRubyFT^S148I) with the blocked over time blue form of the chromophore. X-ray data at a 1.8 Å resolution allowed us to propose a chromophore conformation and its interactions with the neighboring residues. The imidazolidi

SUBMITTER: Boyko KM 

PROVIDER: S-EPMC10178504 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature