IDO1 Promotes the Progression of NSCLC by Regulating the Polarization of M2 Macrophages.

ABSTRACT:

Purpose

Non-small cell lung cancer (NSCLC) is currently a problem in the clinic and in society. Tumor-related macrophages (TAMs) in the tumor microenvironment (TME) play a vital role in the development of NSCLC.

Patients and methods

Bioinformatics was used to analyze the role of Indoleamine 2,3-dioxygenase 1 (IDO1) in NSCLC and the correlation of its expression with CD163 expression. The expression of CD163 and IDO1 was measured by immunohistochemistry, and their colocalization was assessed by immunofluorescence. M2 macrophage polarization was induced, and a coculture model of NSCLC cells and macrophages was established.

Results

Bioinformatics analysis showed that IDO1 promoted the metastasis and differentiation of NSCLC and inhibited DNA repair. Moreover, the express

SUBMITTER: Chen X 

PROVIDER: S-EPMC10178912 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature