Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis.

ABSTRACT: Artificial cells are based on dynamic compartmentalized systems. Thus, remodeling of membrane-bound systems, such as giant unilamellar vesicles, is finding applications beyond biological studies, to engineer cell-mimicking structures. Giant unilamellar vesicle fusion is rapidly becoming an essential experimental step as artificial cells gain prominence in synthetic biology. Several techniques have been developed to accomplish this step, with varying efficiency and selectivity. To date, characterization of vesicle fusion has relied on small samples of giant vesicles, examined either manually or by fluorometric assays on suspensions of small and large unilamellar vesicles. Automation of the detection and characterization of fusion products is now necessary for the screening and optimization

SUBMITTER: Caliari A 

PROVIDER: S-EPMC10179211 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature