A Novel Anti-CD44 Variant 3 Monoclonal Antibody C₄₄Mab-6 Was Established for Multiple Applications.

ABSTRACT: Cluster of differentiation 44 (CD44) promotes tumor progression through the recruitment of growth factors and the acquisition of stemness, invasiveness, and drug resistance. CD44 has multiple isoforms including CD44 standard (CD44s) and CD44 variants (CD44v), which have common and unique functions in tumor development. Therefore, elucidating the function of each CD44 isoform in a tumor is essential for the establishment of CD44-targeting tumor therapy. We have established various anti-CD44s and anti-CD44v monoclonal antibodies (mAbs) through the immunization of CD44v3-10-overexpressed cells. In this study, we established C₄₄Mab-6 (IgG₁, kappa), which recognized the CD44 variant 3-encoded region (CD44v3), as determined via an enzyme-linked immunosorbent assay. C₄₄

SUBMITTER: Suzuki H 

PROVIDER: S-EPMC10179237 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature