Structural Dynamics Predominantly Determine the Adaptability of Proteins to Amino Acid Deletions.

ABSTRACT: The insertion or deletion (indel) of amino acids has a variety of effects on protein function, ranging from disease-forming changes to gaining new functions. Despite their importance, indels have not been systematically characterized towards protein engineering or modification goals. In the present work, we focus on deletions composed of multiple contiguous amino acids (mAA-dels) and their effects on the protein (mutant) folding ability. Our analysis reveals that the mutant retains the native fold when the mAA-del obeys well-defined structural dynamics properties: localization in intrinsically flexible regions, showing low resistance to mechanical stress, and separation from allosteric signaling paths. Motivated by the possibility of distinguishing the features that underlie the adaptabili

SUBMITTER: Banerjee A 

PROVIDER: S-EPMC10179678 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature