Design and Construction of a Synthetic Nanobody Library: Testing Its Potential with a Single Selection Round Strategy.

ABSTRACT: Nanobodies (Nbs) are single domain antibody fragments derived from heavy-chain antibodies found in members of the Camelidae family. They have become a relevant class of biomolecules for many different applications because of several important advantages such as their small size, high solubility and stability, and low production costs. On the other hand, synthetic Nb libraries are emerging as an attractive alternative to animal immunization for the selection of antigen-specific Nbs. Here, we present the design and construction of a new synthetic nanobody library using the phage display technology, following a structure-based approach in which the three hypervariable loops were subjected to position-specific randomization schemes. The constructed library has a clonal diversity of 10⁸

SUBMITTER: Contreras MA 

PROVIDER: S-EPMC10180287 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature