N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21^cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition.

ABSTRACT: Multiple myeloma (MM) is the second most common hematological malignancy. N6-methyladenosine (m⁶A) is the most abundant RNA modification. YTH domain-containing family protein 2 (YTHDF2) recognizes m⁶A-cotaining RNAs and accelerates degradation to regulate cancer progression. However, the role of YTHDF2 in MM remains unclear. We investigated the expression levels and prognostic role of YTHDF2 in MM, and studied the effect of YTHDF2 on MM proliferation and cell cycle. The results showed that YTHDF2 was highly expressed in MM and was an independent prognostic factor for MM survival. Silencing YTHDF2 suppressed cell proliferation and caused the G₁/S phase cell cycle arrest. RNA immunoprecipitation (RIP) and m⁶A-RIP (MeRIP) revealed that YTHDF2 accele

SUBMITTER: Liu R 

PROVIDER: S-EPMC10181929 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature