Non-destructive collection and metabarcoding of arthropod environmental DNA remained on a terrestrial plant.

ABSTRACT: Reliable survey of arthropods is a crucial for their conservation, community ecology, and pest control on terrestrial plants. However, efficient and comprehensive surveys are hindered by challenges in collecting arthropods and identifying especially small species. To address this issue, we developed a non-destructive environmental DNA (eDNA) collection method termed "plant flow collection" to apply eDNA metabarcoding to terrestrial arthropods. This involves spraying distilled or tap water, or using rainfall, which eventually flows over the surface of the plant, and is collected in a container that is set at the plant base. DNA is extracted from collected water and a DNA barcode region of cytochrome c oxidase subunit I (COI) gene is amplified and sequenced using a high-throughput Illumina M

SUBMITTER: Yoneya K 

PROVIDER: S-EPMC10182007 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature