Rotary properties of hybrid F₁-ATPases consisting of subunits from different species.

ABSTRACT: F₁-ATPase (F₁) is an ATP-driven rotary motor protein ubiquitously found in many species as the catalytic portion of F_oF₁-ATP synthase. Despite the highly conserved amino acid sequence of the catalytic core subunits: α and β, F₁ shows diversity in the maximum catalytic turnover rate V_max and the number of rotary steps per turn. To study the design principle of F₁, we prepared eight hybrid F₁s composed of subunits from two of three genuine F₁s: thermophilic Bacillus PS3 (TF₁), bovine mitochondria (bMF₁), and Paracoccus denitrificans (PdF₁), differing in the V_max and the number of rotary steps. The V_max

SUBMITTER: Watanabe RR 

PROVIDER: S-EPMC10182284 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature