Evaluation of in vitro and in vivo personalized cancer treatment assays for oral squamous cell carcinoma.

ABSTRACT:

Background

Oral squamous cell carcinoma (OSCC) is a common cancer with a high heterogeneity and few approved treatments. OSCC is one of the least explored areas for precision oncology. In this study, we aimed to test the reliability of our three established rapid cancer systemic treatment-testing assays: human tumour-derived matrix (Myogel)-coated well-plates, zebrafish xenografts, and 3D microfluidic chips.

Methods

Chemo-, radio- and targeted-therapy testing in Myogel-coated wells and zebrafish xenografts was conducted nine times using five samples; two primary and three metastatic lymph node samples from three OSCC patients. Peripheral blood mononuclear cells (PBMNCs) were isolated from the patients' blood. The response of the tumour cells to radio-, chemo-, and targeted t

SUBMITTER: Wahbi W 

PROVIDER: S-EPMC10182324 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature