TSF/FHA induces osteogenic differentiation of Mc3t3 cells via Pygo2 dependent Wnt/β-catenin signaling pathway.

ABSTRACT:

Objectives

This study aimed to investigate whether tussah silk fibroin (TSF)/fluoridated hydroxyapatite (FHA) can promote osteogenic differentiation of Mc3t3 cells and explore the role of Wnt/β-catenin signaling in this process.

Methods

TSF/FHA was gained via freeze drying technique and cyclic phosphate immersion method. The relative expression levels of bone-related genes and proteins of Mc3t3 cells seeded on different materials were examined by RT-qPCR and Western blotting. Knockdown or overexpression of Pygo2 in Mc3t3 cells was achieved using lentiviral transfection. Cell proliferation, the expression of bone-related genes and proteins were subsequently examined. Animal experiment was also performed to observe the osteogenesis effect.

Results

Different ratios of fl

SUBMITTER: Dong W 

PROVIDER: S-EPMC10182493 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature