Multicellular network analysis of melanoma heterogeneity by scRNA-seq.

ABSTRACT:

Objective

Melanoma neoplasia is a complicated process. Not only melanocytes are involved, stromal cells and immune cells would also regulate cancer development. However, the cell type composition and tumor immune microenvironment of melanoma are poorly understood.

Method

Here we report a map of the cellular landscape of human melanoma via analyzing published single-cell RNA sequencing (scRNA-seq) dataset. Transcriptional profiles of 4645 cells obtained from 19 melanoma tissues were dissected.

Result

Using gene expression patterns and flow cytometry, 8 discrete cell populations were identified including endothelial cells (ECs), cancer related fibroblasts (CAFs), macrophages, B cells, T cells (NK cells), memory T cells (MTCs), melanocytes and podocytes. By constructing

SUBMITTER: Li S 

PROVIDER: S-EPMC10182521 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature