Comparing genomes recovered from time-series metagenomes using long- and short-read sequencing technologies.

ABSTRACT:

Background

Over the past years, sequencing technologies have expanded our ability to examine novel microbial metabolisms and diversity previously obscured by isolation approaches. Long-read sequencing promises to revolutionize the metagenomic field and recover less fragmented genomes from environmental samples. Nonetheless, how to best benefit from long-read sequencing and whether long-read sequencing can provide recovered genomes of similar characteristics as short-read approaches remains unclear.

Results

We recovered metagenome-assembled genomes (MAGs) from the free-living fraction at four-time points during a spring bloom in the North Sea. The taxonomic composition of all MAGs recovered was comparable between technologies. However, differences consisted of higher sequenci

SUBMITTER: Orellana LH 

PROVIDER: S-EPMC10182627 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature