Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern.

ABSTRACT:

Background

Liver metastasis is a poor prognostic factor for treatment of advanced cutaneous melanoma with either immunotherapy or targeted therapies. In this study we focused on NRAS mutated melanoma, a cohort with high unmet clinical need.

Methods

WT31 melanoma was repeatedly passaged over the liver after intravenous injections five times generating the subline WT31_P5IV. The colonization of target organs, morphology, vascularization and the gene expression profiles of metastases were analyzed.

Results

After intravenous injection lung metastasis was significantly decreased and a trend towards increased liver metastasis was detected for WT31_P5IV as compared to parental WT31. Besides, the ratio of lung to liver metastases was significantly smaller. Histology of lung m

SUBMITTER: Dietsch B 

PROVIDER: S-EPMC10182637 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature