The pattern of histone H3 epigenetic posttranslational modifications is regulated by the VRK1 chromatin kinase.

ABSTRACT:

Background

Dynamic chromatin remodeling is associated with changes in the epigenetic pattern of histone acetylations and methylations required for processes based on dynamic chromatin remodeling and implicated in different nuclear functions. These histone epigenetic modifications need to be coordinated, a role that may be mediated by chromatin kinases such as VRK1, which phosphorylates histones H3 and H2A.

Methods

The effect of VRK1 depletion and VRK1 inhibitor, VRK-IN-1, on the acetylation and methylation of histone H3 in K4, K9 and K27 was determined under different conditions, arrested or proliferating cells, in A549 lung adenocarcinoma and U2OS osteosarcoma cells.

Results

Chromatin organization is determined by the phosphorylation pattern of histones mediated by d

SUBMITTER: Monte-Serrano E 

PROVIDER: S-EPMC10182654 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature