A comparison of phenotypic and WGS drug susceptibility testing in <i>Mycobacterium tuberculosis</i> isolates from the Republic of Korea.

ABSTRACT:

Background

WGS has significant potential to help tackle the major public health problem of TB. The Republic of Korea has the third highest rates of TB of all Organisation for Economic Cooperation and Development countries but there has been very limited use of WGS in TB to date.

Objectives

A retrospective comparison of Mycobacterium tuberculosis (MTB) clinical isolates from 2015 to 2017 from two centres in the Republic of Korea using WGS to compare phenotypic drug susceptibility testing (pDST) and WGS drug susceptibility predictions (WGS-DSP).

Methods

Fifty-seven MTB isolates had DNA extracted and were sequenced using the Illumina HiSeq platform. The WGS analysis was performed using bwa mem, bcftools and IQ-Tree; resistance markers were identified using TB prof

SUBMITTER: Lee SH 

PROVIDER: S-EPMC10182733 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature