Robust microtubule dynamics facilitate low-tension kinetochore detachment in metaphase.

ABSTRACT: During mitosis, sister chromatids are stretched apart at their centromeres via their attachment to oppositely oriented kinetochore microtubules. This stretching generates inwardly directed tension across the separated sister centromeres. The cell leverages this tension signal to detect and then correct potential errors in chromosome segregation, via a mechanical tension signaling pathway that detaches improperly attached kinetochores from their microtubules. However, the sequence of events leading up to these detachment events remains unknown. In this study, we used microfluidics to sustain and observe low-tension budding yeast metaphase spindles over multiple hours, allowing us to elucidate the tension history prior to a detachment event. We found that, under conditions in which kinetocho

SUBMITTER: Parmar S 

PROVIDER: S-EPMC10182774 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature