M⁶A eraser ALKBH5 mitigates the apoptosis of cardiomyocytes in ischemia reperfusion injury through m⁶A/SIRT1 axis.

ABSTRACT: Recent studies have shown that the potential regulatory role of N⁶-methyladenine (m⁶A) modification may affect the occurrence and development of various cardiovascular diseases. However, the regulatory mechanism of m⁶A modification on myocardial ischemia reperfusion injury (MIRI) is rarely reported. A mouse model of myocardial ischemia reperfusion (I/R) was established by ligation and perfusion of the left anterior descending coronary artery, and a cellular model of hypoxia/reperfusion (H/R) was conducted in cardiomyocytes (CMs). We found that the protein expression of ALKBH5 in myocardial tissues and cells were decreased, accompanied by increased m⁶A modification level. Overexpression of ALKBH5 significantly inhibited H/R-induced oxidative stres

SUBMITTER: Liu L 

PROVIDER: S-EPMC10183170 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature