Novel model of cortical-meningeal organoid co-culture system improves human cortical brain organoid cytoarchitecture.

ABSTRACT: Human cortical organoids (hCOs), derived from human induced pluripotent stem cells (iPSCs), provide a platform to interrogate mechanisms of human brain development and diseases in complex three- dimensional tissues. However, current hCO development methods lack important non-neural tissues, such as the surrounding meningeal layer, that have been shown to be essential for normal corticogenesis and brain development. Here, we first generated hCOs from a single rosette to create more homogenous organoids with consistent size around 250 µm by day 5. We then took advantage of a 3D co-culture system to encapsulate brain organoids with a thin layer of meningeal cells from the very early stages of cortical development. Immunostaining analysis was performed to display different cortical layer marke

SUBMITTER: Jalilian E 

PROVIDER: S-EPMC10183460 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature