Establishment of an orthotopic model of lung cancer by transthoracic lung puncture using tumor fragments.

ABSTRACT:

Background

Orthotopic models of lung cancer have been widely utilized, and the purpose of this study was to demonstrate the viability of our proposed modified modeling approach.

Methods

A total of 50 female BALB/c mice were implanted with 1×1×1 mm fragments of a tumor sample into the left lung lobe. After 2 months of observation, the mice were humanely euthanized through CO₂ inhalation. The macroscopic specimens were photographed, and the most representative neoplastic lesions were collected for histological analysis. Small-animal positron emission tomography/computed tomography (PET/CT) scans were conducted on 6 randomly selected mice.

Results

Local tumor formation, ipsilateral thoracic tissue infiltration, the contralateral chest wall, right lung metastas

SUBMITTER: Gan W 

PROVIDER: S-EPMC10183487 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature