KNO1-mediated autophagic degradation of the Bloom syndrome complex component RMI1 promotes homologous recombination.

ABSTRACT: Homologous recombination (HR) is a key DNA damage repair pathway that is tightly adjusted to the state of a cell. A central regulator of homologous recombination is the conserved helicase-containing Bloom syndrome complex, renowned for its crucial role in maintaining genome integrity. Here, we show that in Arabidopsis thaliana, Bloom complex activity is controlled by selective autophagy. We find that the recently identified DNA damage regulator KNO1 facilitates K63-linked ubiquitination of RMI1, a structural component of the complex, thereby triggering RMI1 autophagic degradation and resulting in increased homologous recombination. Conversely, reduced autophagic activity makes plants hypersensitive to DNA damage. KNO1 itself is also controlled at the level of proteolysis, in this case medi

SUBMITTER: Chen P 

PROVIDER: S-EPMC10183828 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature