Near-equilibrium glycolysis supports metabolic homeostasis and energy yield.

ABSTRACT: Glycolysis plays a central role in producing ATP and biomass. Its control principles, however, remain incompletely understood. Here, we develop a method that combines ²H and ¹³C tracers to determine glycolytic thermodynamics. Using this method, we show that, in conditions and organisms with relatively slow fluxes, multiple steps in glycolysis are near to equilibrium, reflecting spare enzyme capacity. In Escherichia coli, nitrogen or phosphorus upshift rapidly increases the thermodynamic driving force, deploying the spare enzyme capacity to increase flux. Similarly, respiration inhibition in mammalian cells rapidly increases both glycolytic flux and the thermodynamic driving force. The thermodynamic shift allows flux to increase with only small metabolite concentration

SUBMITTER: Park JO 

PROVIDER: S-EPMC10184052 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature