Bacterial extracellular vesicles repress the vascular protective factor RNase1 in human lung endothelial cells.

ABSTRACT:

Background

Sepsis is one of the leading causes of death worldwide and characterized by blood stream infections associated with a dysregulated host response and endothelial cell (EC) dysfunction. Ribonuclease 1 (RNase1) acts as a protective factor of vascular homeostasis and is known to be repressed by massive and persistent inflammation, associated to the development of vascular pathologies. Bacterial extracellular vesicles (bEVs) are released upon infection and may interact with ECs to mediate EC barrier dysfunction. Here, we investigated the impact of bEVs of sepsis-related pathogens on human EC RNase1 regulation.

Methods

bEVs from sepsis-associated bacteria were isolated via ultrafiltration and size exclusion chromatography and used for stimulation of human lung microvasc

SUBMITTER: Laakmann K 

PROVIDER: S-EPMC10184351 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature