ABSTRACT: In this study, we sequenced and reported the complete mitochondrial genomes of Kusala populi for the first time. The complete mitochondrial genome was registered in GenBank with accession number NC_064377 as the first complete mitogenome of the genus Kusala. The circular mitochondrial genome length is 15,402 bp, with nucleotide composition A (41.8%), C (11.4%), G (9.2%), T (37.6%), A + T (79.4%), and C + G (20.6%), comprising 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a D-loop region. All protein-coding genes were encoded by the H-strand, except for 4 genes (nad5, nad4, nad4L, nad1). 8 tRNA genes (tRNA-Gln, tRNA-Cys, tRNA-Tyr, tRNA-Phe, tRNA-His, tRNA-Pro, tRNA-Leu, tRNA-Val) and
