In Vivo Capsid Engineering of Bacteriophages for Oriented Surface Conjugation.

ABSTRACT: The current state-of-the-art in bacteriophage (phage) immobilization onto magnetic particles is limited to techniques that are less expensive and/or facile but nonspecific or those that are more expensive and/or complicated but ensure capsid-down orientation of the phages, as necessary to preserve infectivity and performance in subsequent applications (e.g., therapeutics, detection). These cost, complexity, and effectiveness limitations constitute the major hurdles that limit the scale-up of phage-based strategies and thus their accessibility in low-resource settings. Here, we report a plasmid-based technique that incorporates a silica-binding protein, L2, into the T7 phage capsid, during viral assembly, with and without inclusion of a flexible linker peptide, allowing for targeted binding

SUBMITTER: Hufziger KA 

PROVIDER: S-EPMC10184791 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature