MitoTNT: Mitochondrial Temporal Network Tracking for 4D live-cell fluorescence microscopy data.

ABSTRACT: Mitochondria form a network in the cell that rapidly changes through fission, fusion, and motility. Dysregulation of this four-dimensional (4D: x,y,z,time) network is implicated in numerous diseases ranging from cancer to neurodegeneration. While lattice light-sheet microscopy has recently made it possible to image mitochondria in 4D, quantitative analysis methods for the resulting datasets have been lacking. Here we present MitoTNT, the first-in-class software for Mitochondrial Temporal Network Tracking in 4D live-cell fluorescence microscopy data. MitoTNT uses spatial proximity and network topology to compute an optimal tracking assignment. To validate the accuracy of tracking, we created a reaction-diffusion simulation to model mitochondrial network motion and remodeling events. We foun

SUBMITTER: Wang Z 

PROVIDER: S-EPMC10184899 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature