Partitioning and subsampling statistics in compartment-based quantification methods.

ABSTRACT: The precision of compartment-based quantification methods is subject to multiple effects, of which partitioning and subsampling play a major role. Partitioning is the process of aliquoting the sample liquid and consequently the contained target molecules, whereas subsampling denotes the fact that usually only a portion of a sample is analyzed. In this work, we present a detailed statistical description comprising the effects of partitioning and subsampling on the relative uncertainty of the test result. We show that the state-of-the-art binomial model does not provide accurate results for the level of subsampling present when analyzing the nucleic acid content of single specific cells. Hence, in this work we address partitioning and subsampling effects separately and subsequently combine t

SUBMITTER: Loskyll M 

PROVIDER: S-EPMC10184943 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature