Transcriptome network analysis implicates CX3CR1-positive type 3 dendritic cells in non-infectious uveitis.

ABSTRACT:

Background

Type I interferons (IFNs) promote the expansion of subsets of CD1c+ conventional dendritic cells (CD1c+ DCs), but the molecular basis of CD1c+ DCs involvement in conditions not associated without elevated type I IFNs remains unclear.

Methods

We analyzed CD1c+ DCs from two cohorts of non-infectious uveitis patients and healthy donors using RNA-sequencing followed by high-dimensional flow cytometry to characterize the CD1c+ DC populations.

Results

We report that the CD1c+ DCs pool from patients with non-infectious uveitis is skewed toward a gene module with the chemokine receptor CX3CR1 as the key hub gene. We confirmed these results in an independent case-control cohort and show that the disease-associated gene module is not mediated by type I IFNs. A

SUBMITTER: Hiddingh S 

PROVIDER: S-EPMC10185339 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature