Dataset Information

A peleg modeling of water absorption in cold plasma-treated Chickpea (Cicer arietinum L.) cultivars.

ABSTRACT: Plasma processing appears to be the mainstay of food preservation in the present day due to its effectiveness in controlling microorganisms at low temperatures. Legumes are usually soaked before cooking. Six chickpea varieties (Kripa, Virat, Vishal, Vijay, Digvijay, and Rajas) were soaked in distilled water at room temperature, and Peleg model was fitted after plasma treatment. Cold plasma treatment was used at 40, 50 and 60 Watt with exposure times of 10, 15 and 20 min. K₁ (Peleg rate constant) consistently decreased from 32.3 to 4.3 × 10^-3 (h % - 1) for all six chickpea cultivars, indicating an increased water absorption rate with increasing plasma power and treatment time. It was lowest in 60 W 20 min plasma treatment in Virat cultivar. K₂ (Peleg capacity constant) ranged from 9.4 to 12 × 10^-3 (h % - 1) for all six chickpea cultivars. Thus, plasma treatment showed no effect on water uptake capacity (K₂), as it did not increase or decrease consistently with increasing plasma power and treatment time. Fitting the Peleg model successfully revealed the correlation between the water absorption of chickpea cultivars. The model fit ranged from R² ≥ 0.9873 to 0.9981 for all six chickpea cultivars.

SUBMITTER: Pathan FL

PROVIDER: S-EPMC10185522 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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A peleg modeling of water absorption in cold plasma-treated Chickpea (Cicer arietinum L.) cultivars.

Pathan F L FL Trimukhe A M AM Deshmukh R R RR Annapure U S US

Scientific reports 20230515 1

Plasma processing appears to be the mainstay of food preservation in the present day due to its effectiveness in controlling microorganisms at low temperatures. Legumes are usually soaked before cooking. Six chickpea varieties (Kripa, Virat, Vishal, Vijay, Digvijay, and Rajas) were soaked in distilled water at room temperature, and Peleg model was fitted after plasma treatment. Cold plasma treatment was used at 40, 50 and 60 Watt with exposure times of 10, 15 and 20 min. K<sub>1</sub> (Peleg rat ...[more]

PMID: 37188721

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Project description:BackgroundCold stress at reproductive phase in susceptible chickpea (Cicer arietinum L.) leads to pollen sterility induced flower abortion. The tolerant genotypes, on the other hand, produce viable pollen and set seed under cold stress. Genomic information on pollen development in cold-tolerant chickpea under cold stress is currently unavailable.ResultsDDRT-PCR analysis was carried out to identify anther genes involved in cold tolerance in chickpea genotype ICC16349 (cold-tolerant). A total of 9205 EST bands were analyzed. Cold stress altered expression of 127 ESTs (90 up-regulated, 37 down-regulated) in anthers, more than two third (92) of which were novel with unknown protein identity and function. Remaining about one third (35) belonged to several functional categories such as pollen development, signal transduction, ion transport, transcription, carbohydrate metabolism, translation, energy and cell division. The categories with more number of transcripts were carbohydrate/triacylglycerol metabolism, signal transduction, pollen development and transport. All but two transcripts in these categories were up-regulated under cold stress. To identify time of regulation after stress and organ specificity, expression levels of 25 differentially regulated transcripts were also studied in anthers at six time points and in four organs (anthers, gynoecium, leaves and roots) at four time points.ConclusionsLimited number of genes were involved in regulating cold tolerance in chickpea anthers. Moreover, the cold tolerance was manifested by up-regulation of majority of the differentially expressed transcripts. The anthers appeared to employ dual cold tolerance mechanism based on their protection from cold by enhancing triacylglycerol and carbohydrate metabolism; and maintenance of normal pollen development by regulating pollen development genes. Functional characterization of about two third of the novel genes is needed to have precise understanding of the cold tolerance mechanisms in chickpea anthers.

Project description:BackgroundChickpea (Cicer arietinum L.) is the second most widely grown pulse and drought (limiting water) is one of the major constraints leading to about 40-50% yield losses annually. Dehydration responsive element binding proteins (DREBs) are important plant transcription factors that regulate the expression of many stress-inducible genes and play a critical role in improving the abiotic stress tolerance. Transgenic chickpea lines harbouring transcription factor, Dehydration Responsive Element-Binding protein 1A from Arabidopsis thaliana (AtDREB1a gene) driven by stress inducible promoter rd29a were developed, with the intent of enhancing drought tolerance in chickpea. Performance of the progenies of one transgenic event and control were assessed based on key physiological traits imparting drought tolerance such as plant water relation characteristics, chlorophyll retention, photosynthesis, membrane stability and water use efficiency under water stressed conditions.ResultsFour transgenic chickpea lines harbouring stress inducible AtDREB1a were generated with transformation efficiency of 0.1%. The integration, transmission and regulated expression were confirmed by Polymerase Chain Reaction (PCR), Southern Blot hybridization and Reverse Transcriptase polymerase chain reaction (RT-PCR), respectively. Transgenic chickpea lines exhibited higher relative water content, longer chlorophyll retention capacity and higher osmotic adjustment under severe drought stress (stress level 4), as compared to control. The enhanced drought tolerance in transgenic chickpea lines were also manifested by undeterred photosynthesis involving enhanced quantum yield of PSII, electron transport rate at saturated irradiance levels and maintaining higher relative water content in leaves under relatively severe soil water deficit. Further, lower values of carbon isotope discrimination in some transgenic chickpea lines indicated higher water use efficiency. Transgenic chickpea lines exhibiting better OA resulted in higher seed yield, with progressive increase in water stress, as compared to control.ConclusionsBased on precise phenotyping, involving non-invasive chlorophyll fluorescence imaging, carbon isotope discrimination, osmotic adjustment, higher chlorophyll retention and membrane stability index, it can be concluded that AtDREB1a transgenic chickpea lines were better adapted to water deficit by modifying important physiological traits. The selected transgenic chickpea event would be a valuable resource that can be used in pre-breeding or directly in varietal development programs for enhanced drought tolerance under parched conditions.

Dataset Information

A peleg modeling of water absorption in cold plasma-treated Chickpea (Cicer arietinum L.) cultivars.

Publications

A peleg modeling of water absorption in cold plasma-treated Chickpea (Cicer arietinum L.) cultivars.

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