An effective validation of analytical method for determination of a polar complexing agent: the illustrative case of cytotoxic bleomycin.

ABSTRACT: The effectiveness of highly polar agents in cancer treatment is well recognized, but their physicochemical properties make their analytical determination a demanding task. Their analysis requires peculiar sample preparation and chromatographic separation, which heavily impacts the precision of such an analytical method. As a case study, we chose a polar cytotoxic bleomycin, which is a mixture of complexing congeners with relatively high molecular mass, a fact that creates an added challenge in regard to its detection via electrospray mass spectrometry. These issues combined lead to a deprived method performance, so the aim of this study is manifold, i.e., to optimize, validate, and establish quality performance measures for determination of bleomycin in pharmaceutical and biological specim

SUBMITTER: Plesnik H 

PROVIDER: S-EPMC10185589 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature