Morphogenetic proteins SpoVID and SafA form a complex during assembly of the Bacillus subtilis spore coat.

ABSTRACT: During endospore formation in Bacillus subtilis, over two dozen polypeptides are assembled into a multilayered structure known as the spore coat, which protects the cortex peptidoglycan (PG) and permits efficient germination. In the initial stages of coat assembly a protein known as CotE forms a ring around the forespore. A second morphogenetic protein, SpoVID, is required for maintenance of the CotE ring during the later stages, when most of proteins are assembled into the coat. Here, we report on a protein that appears to associate with SpoVID during the early stage of coat assembly. This protein, which we call SafA for SpoVID-associated factor A, is encoded by a locus previously known as yrbA. We confirmed the results of a previous study that showed safA mutant spores have defective coa

SUBMITTER: Ozin AJ 

PROVIDER: S-EPMC101864 | biostudies-literature | 2000 Apr

REPOSITORIES: biostudies-literature