Different iPSC-derived neural stem cells shows various spectrums of spontaneous differentiation during long term cultivation.

ABSTRACT:

Introduction

Culturing of human neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSC) is a promising area of research, as these cells have the potential to treat a wide range of neurological, neurodegenerative and psychiatric diseases. However, the development of optimal protocols for the production and long-term culturing of NSCs remains a challenge. One of the most important aspects of this problem is to determine the stability of NSCs during long-term in vitro passaging. To address this problem, our study was aimed at investigating the spontaneous differentiation profile in different iPSC-derived human NSCs cultures during long-term cultivation using.

Methods

Four different IPSC lines were used to generate NSC and spontaneously differentiated neural

SUBMITTER: Galiakberova AA 

PROVIDER: S-EPMC10186475 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature