The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases.

ABSTRACT:

Introduction

Biofilm production is an important yet currently overlooked aspect of diagnostic microbiology that has implications for antimicrobial stewardship. In this study, we aimed to validate and identify additional applications of the BioFilm Ring Test® (BRT) for Pseudomonas aeruginosa (PA) isolates from patients with bronchiectasis (BE).

Materials and methods

Sputa were collected from BE patients who had at least one PA positive culture in the previous year. We processed the sputa to isolate both mucoid and non-mucoid PA, and determined their susceptibility pattern, mucA gene status, and presence of ciprofloxacin mutations in QRDR genes. The Biofilm production index (BPI) was obtained at 5 and 24 hours. Biofilms were imaged using Gram staining.

Results

We collec

SUBMITTER: Fernandez-Barat L 

PROVIDER: S-EPMC10187140 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature