Genetic manipulation of candidate phyla radiation bacteria provides functional insights into microbial dark matter.

ABSTRACT: The study of bacteria has yielded fundamental insights into cellular biology and physiology, biotechnological advances and many therapeutics. Yet due to a lack of suitable tools, the significant portion of bacterial diversity held within the candidate phyla radiation (CPR) remains inaccessible to such pursuits. Here we show that CPR bacteria belonging to the phylum Saccharibacteria exhibit natural competence. We exploit this property to develop methods for their genetic manipulation, including the insertion of heterologous sequences and the construction of targeted gene deletions. Imaging of fluorescent protein-labeled Saccharibacteria provides high spatiotemporal resolution of phenomena accompanying epibiotic growth and a transposon insertion sequencing genome-wide screen reveals the cont

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10187176 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature