CTCF-mediated insulation and chromatin environment modulate <i>Car5b</i> escape from X inactivation.

ABSTRACT:

Background

The number and escape levels of genes that escape X chromosome inactivation (XCI) in female somatic cells vary among tissues and cell types, potentially contributing to specific sex differences. Here we investigate the role of CTCF, a master chromatin conformation regulator, in regulating escape from XCI. CTCF binding profiles and epigenetic features were systematically examined at constitutive and facultative escape genes using mouse allelic systems to distinguish the inactive X (Xi) and active X (Xa) chromosomes.

Results

We found that escape genes are located inside domains flanked by convergent arrays of CTCF binding sites, consistent with the formation of loops. In addition, strong and divergent CTCF binding sites often located at the boundaries between escape

SUBMITTER: Fang H 

PROVIDER: S-EPMC10187265 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature