Self-assembly of DNA nanostructures in different cations.

ABSTRACT: The programmable nature of DNA allows the construction of custom-designed static and dynamic nanostructures, and assembly conditions typically require high concentrations of magnesium ions which restricts their applications. In other solution conditions tested for DNA nanostructure assembly, only a limited set of divalent and monovalent ions have been used so far (typically Mg ²⁺ and Na ⁺ ). Here, we investigate the assembly of DNA nanostructures in a wide variety of ions using nanostructures of different sizes: a double-crossover motif (76 bp), a three-point-star motif (∼134 bp), a DNA tetrahedron (534 bp) and a DNA origami triangle (7221 bp). We show successful assembly of a majority of these structures in Ca ²⁺ , Ba ²⁺ , Na ⁺ , K

SUBMITTER: Rodriguez A 

PROVIDER: S-EPMC10187274 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature