Genetic control of the error-prone repair of a chromosomal double-strand break with 5' overhangs in yeast.

ABSTRACT: A targeted double-strand break introduced into the genome of Saccharomyces cerevisiae is repaired by the relatively error-prone nonhomologous-end joining (NHEJ) pathway when homologous recombination is not an option. A ZFN cleavage site was inserted out-of-frame into the LYS2 locus of a haploid yeast strain to study the genetic control of NHEJ when the ends contain 5' overhangs. Repair events that destroyed the cleavage site were identified either as Lys ⁺ colonies on selective medium or as surviving colonies on rich medium. Junction sequences in Lys ⁺ events solely reflected NHEJ and were influenced by the nuclease activity of Mre11 as well as by the presence/absence of the NHEJ-specific polymerase Pol4 and the translesion-synthesis DNA polymerases Pol

SUBMITTER: Shaltz S 

PROVIDER: S-EPMC10187297 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature