Cell-Surface Targeting of Fluorophores in Drosophila for Rapid Neuroanatomy Visualization.

ABSTRACT: Visualizing neuronal anatomy often requires labor-intensive immunohistochemistry on fixed and dissected brains. To facilitate rapid anatomical staining in live brains, we used genetically targeted membrane tethers that covalently link fluorescent dyes for in vivo neuronal labeling. We generated a series of extracellularly trafficked small-molecule tethering proteins, HaloTag-CD4 (Kirk et al. Front. Neurosci. 2021, 15, 754027) and SNAP_f-CD4, which directly label transgene-expressing cells with commercially available ligand-substituted fluorescent dyes. We created stable transgenic Drosophila reporter lines, which express extracellular HaloTag-CD4 and SNAP_f-CD4 with LexA and Gal4 drivers. Expressing these enzymes in live Drosophila bra

SUBMITTER: Kirk MJ 

PROVIDER: S-EPMC10187464 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature