Evaluation of T and B cell immunophenotyping and antibody response post Covid-19 vaccination

ABSTRACT:

Purpose

To evaluate T and B cell subsets and IgG antibodies in response to SARS-CoV-2 post COVID-19 vaccination.

Methods

A total of 50 healthy adults (18–60 years) receiving anti-SARS-CoV-2 vaccination (COVISHIELD) were recruited for the study. Blood samples were collected from participants at 3 time points; just before vaccination (Visit 0, V0), just before booster dose (Visit 1, V1) and 6th month after 1st dose (Visit 2, V2). Peripheral blood mononuclear cell isolation was done and evaluated for T and B cell subsets by Flow cytometry. Quantitative determination of IgG antibodies to SARS-CoV-2 was done by Chemiluminescence immunoassay in all samples. Final data for all three visits was available for 37 participants who remained healthy. Ethics approval was obtained from

SUBMITTER: Chadha R 

PROVIDER: S-EPMC10187542 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature