Genome-Wide Libraries for Protozoan Pathogen Drug Target Screening Using Yeast Surface Display.

ABSTRACT: The lack of genetic tools to manipulate protozoan pathogens has limited the use of genome-wide approaches to identify drug or vaccine targets and understand these organisms' biology. We have developed an efficient method to construct genome-wide libraries for yeast surface display (YSD) and developed a YSD fitness screen (YSD-FS) to identify drug targets. We show the efficacy of our method by generating genome-wide libraries for Trypanosoma brucei, Trypanosoma cruzi, and Giardia lamblia parasites. Each library has a diversity of ∼10⁵ to 10⁶ clones, representing ∼6- to 30-fold of the parasite's genome. Nanopore sequencing confirmed the libraries' genome coverage with multiple clones for each parasite gene. Western blot and imaging analysis confirme

SUBMITTER: Heslop R 

PROVIDER: S-EPMC10187560 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature