EGFR-Targeted ImmunoPET of UMUC3 Orthotopic Bladder Tumors.

ABSTRACT:

Purpose

Immuno-positron emission tomography (immunoPET) combines the specificity of an antibody with the sensitivity of PET to image dysregulated pathways in cancer. This study examines the performance of immunoPET using the radioimmunoconjugate [⁸⁹Zr]Zr-DFO-Panitumumab to detect epidermal growth factor receptor (EGFR) expression in an orthotopic model of bladder cancer (BCa).

Procedures

Expression and quantification of EGFR receptors were confirmed in four different BCa cell lines. Binding assays validated [⁸⁹Zr]Zr-DFO-Panitumumab specificity for EGFR-expressing UMUC3 BCa cells. Subcutaneous and orthotopic UMUC3 xenografts were then used for PET imaging and ex vivo biodistribution of the radioimmunoconjugate. Control cohorts included non-tumor mice,

SUBMITTER: Hoang TT 

PROVIDER: S-EPMC10187976 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature