Identification of Cutibacterium modestum in Spondylitis by Metagenomics Analysis.

ABSTRACT:

Background/aim

Identifying pathogens with culture-negative pyogenic spondylitis is difficult. Shotgun metagenomic sequencing is an unbiased and culture-free approach in the diagnosis of infectious diseases. There are, however, a variety of contaminating factors that can confound the precision of metagenomic sequencing.

Case report

In a 65-year-old man suffering from culture-negative L3-5 spondylitis, metagenomics was applied to facilitate the diagnosis. The patient underwent percutaneous endoscopic lumbar discectomy. We applied metagenomic sequencing with a robust contamination-free protocol to the bone biopsy. By comparing the abundance for each taxon between the replicates and negative controls, we reliably identified Cutibacterium modestum as having a statistically higher

SUBMITTER: Huang YT 

PROVIDER: S-EPMC10188021 | biostudies-literature | 2023 May-Jun

REPOSITORIES: biostudies-literature